ON THE DETACHMENT OF IRON FROM PSEUDOHEMOGLOBIN

抄録

Green pigment formation from oxyhemoglobin were studied under varied reaction conditions with special reference to its connection with the easily detachable iron content, detachable by the treatment with 0.4 per cent HCl. The results are summarized as follows:
1. The content of easily detachable iron in the pseudohemoglobin produced by the reaction of Hb-KCN-H₂O₂ system was found to be relatively high. In cases with larger amounts of H₂O₂, it should not be overlooked the possible contamination of a few amounts of iron derived from a sort of by-reaction.
2. The content of easily detachable iron in the pseudohemoglobin produced by the reaction of Hb-KCN-ascorbic acid-O₂ system was very low. The fact suggests the differences of the chemical structure between pseudohemoglobin and choleglobin.
3. The reaction products in the system of Hb-K₂CO₃-ascorbic acid-O₂ at higher pH were assumed to be different from the ordinary choleglobin in many points. At least they do not liberate their iron so easily as choleglobin.
4. More than two pigments were suggested, further, to be formed in the present reaction system which seemed chemically different from each other. Among them, one would hardly liberate its iron, the other would, however, more easily. The former seemed to be convertible into the latter in the present reaction process. It may be pointed out additionally that a sort of step-reaction seems to take place even in the presence of KCN.
The present investigation was supported by Prof. K. Kaziro, whom the authers wish to thank.