ON THE REACTION OF PSEUDOTHEMOGLOBIN FORMATION IN THE PRESENCE OF SODIUM DITHIONITE
1954 年 41 巻 2 号 p. 247-254
詳細
1954 年 41 巻 2 号 p. 247-254
1. Much higher amount of pseudohemoglobin could be formed by the reactions of Hb-KCN-Na2S2O4-ascorbic acid-O2 system and of Hb-KCN-Na2S2O4-H2O2 system respectively, both of which were introduced by the present authors, than those obtained by the ordinary reactions of Hb-KCN-ascorbic acid-O2 system or HbO2 KCN-H2O2 system.
2. It may especially be noted that the reaction rate of pseudo-hemoglobin formation in the reaction of Hb-KCN-Na2S2O4-ascorbic acid-O2 system increased with the increase of the concentration of both ascorbic acid and KCN.
3. KOCN was proved to be samely effective as KCN for the reaction of pseudohemoglobin formation.
4. It may be conclusively stated that pseudohemoglobin is derived from hemoglobin of ferrous state.
5. The mechanism of the reaction of pseudohemoglobin formation seems to be much more complicated than has been explained by Barkan.
The present study was supported by Prof. K. Kaziro, for which the author's deep gratitude is expressed.
