IMPROVED METHOD FOR THE PREPARATION OF CRYSTALLINE OXYHEMOGLOBIN FREE FROM CATALASE AND METHEMOGLOBIN
1954 年 41 巻 4 号 p. 401-408
詳細
1954 年 41 巻 4 号 p. 401-408
1. Crystalline preparations of bovine and horse oxyhemoglobin which were free from catalase and methaemoglobin, were obtained.
2. Crystalline bovine oxyhemoglobin was prepared by. means of fractionation with ethanol. Crystalline horse oxyhemoglobin could be obtained merely by dialysis against cold distilled water, but, often with poorer yields and with unsatisfactory reproducibility. In its simplicity, however, Heidelberge is method was proved so far to be most convenient. The adequate conditions of the latter method were carefully studied in the present investigation.
3. The preparation of horse oxyhemoglobin prepared by Heidelberger's method remained fairly stable in its native state, while bovine oxyhemoglobin seemed to have been subjected to some denaturation in its protein part.
4. Contents of N and Fe in the horse hemoglobin prepared by us were found to be 16.5 per cent and 0.34 per cent, respectively.
5. Using the preparations obtained, the optical constants of various hemoglobin derivatives were determined.
Present investigation was undertaken as a part of the program of our laboratory: “ Interrelation between the function of heme-proteins and the structural modifications of their protein parts.”
We wish to thank Profs. K. Kazito and G. Kikuchi for their suggestion and kind advices.
