STUDIES ON AN AMYLASE OF CANDIDA TROPICALIS VAR. JAPONICA

I. MALTASE AND TRANSGLUCOSYLASE ACTIVITIES OF THE AMYLASE

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Amylase of Candida tropicalis var. japonica was inferred to have maltase and transglucosylase activities on the basis of the following experimental results:
Optimum pH's of these three enzyme activities were all 4.0 and activity ratios among them were always essentially constant even if the enzyme preparation was developed on an ion exchange resin column, treated with heat, acid or alkali, or fractionated by electrophoresis, or the yeast cells from which enzyme solutions were obtained were grown on different carbon sources.
The author wishes to express his hearty gratitude to Prof. T. Miwa, Prof. J. Fukumoto and Dr. T. Yamamoto for their kind advices and encouragement, and to Dr. M. A. J ermyn, Wood Textile Research Laboratory, Commonwealth Scientific and Industrial Research Organization, Australia, for his kindness in reviewing this manuscript. The major part of this work was carried out in the laboratory, Institute of Polytechnics, Osaka City University. The expenses of this work were in part defrayed by a Grant in Aid for Scientific Research from the Ministry of Education.