Studies on the Toxic Substances Isolated from Mycobacteria

II. Toxic Glycolipids of Mycobacterium smegmatis, Mycobacterium phlei and Atypical Mycobacteria Strain No. 6 and No. 22

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1. Purified waxes were extracted from Mycobacterium smegmatis, Mycobacterium phlei and atypical mycobacteria strain No. 6 and No. 22 in the yields of 2 to 4 per cent of bacilli.
2. Toxic lipids were fractionated from purified wax by column chromatographies on silica gel, silicic acid and Florisil and their yields were 0.3 to 2.2 per cent of purified wax.
3. These glycolipids were active at 0.01mg. dose levels in the toxicity to mice.
4. Lipid moiety of these glycolipids were mycolic acids. Mycolic acids of Mycobacterium smegmatis and Mycobacterium phleiii were different from mycolic acid of tubercle bacilli and assumed to have the formula C₆₇H₁₃₄O₆.
5. Water soluble fraction of these toxic glycolipids was trehalose.
The authors express their deep gratitude to Dr. H. Oura, School of Pharmacy, Toyama University, for his helpful suggestion and Dr. A. Yasuda and Dr. Y. Fujiwara for cultivation of bacilli. The authors are indebted to Mrs. S. Matsuba for elementary analysis, and to Mr. K. Hikita for spectral measurement.