Chemical Bleaching Improves the Fluorescence Detection Efficiency of Fungi Possessing Dark-Brown Pigments

抄録

Fungi possessing dark-brown pigments such as plant melanin are difficult to detect by fluorescence microscopic techniques. Pretreatment of the pigmented fungi with oxides or chlorides improved the detection efficiency by the fluorescence microscopic technique. Chemical bleaching was effective for spores of all the fungi studied. The fluorescence intensity of probes for cytoplasmic membranes, nucleic acids, and esterases stained to the spores increased more than ten times by this pretreatment, and became detectable not only with an electronic camera but also with the eye.