An attempt to cell differentiation with EL-M3 and ES-R1-EGFP B2/EGFP cell lines using uterus- and fallopian tube-derived feeder cells

抄録

Various studies have been conducted on regenerative medicine, by which iPS cells are differentiated to produce tissues and organs to be transplanted into the injured sites of patients. Results obtained with mouse ES cells are likely to be applied to studies on iPS and ES cell differentiation cultures. Mouse MEF (Mouse Fibroblast Feeder) cells are utilized as feeder cells. Since ES cells are derived from the reproductive organs, such as the uterus and fallopian tube, MEF cells should be applied as feeder cells to ES cell differentiation culture. Therefore, uterusand fallopian tube-derived feeder cells were prepared to examine the cardiomyocyte differentiation of ES cells. EL M3 and ES-R1-EGFP B2/EGFP cells were uterus- and fallopian tube-derived cells, respectively. They showed higher cardiomyocyte differentiation rates than the control MEF cells, whereas other fallopian tube-derived cells showed lower rates, suggesting the contamination of heterogeneous cells. Thus, the uterus- and fallopian tubederived cells also served as feeder cells, some of which were more suitable for cardiomyocyte differentiation than commercially - available feeder cells, MEF cells.