バイオインテグレーション学会誌
Online ISSN : 2186-2923
7 巻, 1 号
選択された号の論文の10件中1~10を表示しています
  • 川上 紗和子, 塩田 真, 今北 千春, 藤森 達也, 春日井 昇平
    2017 年 7 巻 1 号 p. 27-31
    発行日: 2017年
    公開日: 2020/07/18
    ジャーナル オープンアクセス
    Implant therapy has become a common treatment standard of partial and total edentulism during the past quarter-century. In clinical examination, Multi Detector-row Computed Tomography or Corn Beam Computed Tomography is generally used to identify anatomical features of patient jaws that needs implant therapy. However, some problems should be considered such as radiation exposure and impossible of real-time display. Optical Coherence Tomography (OCT) is a real-time display technique, which is characterized by no contact, no invasion and no radiation exposure to realize images. The aim of this study was to investigate the potential for real-time display of OCT to image implant sockets. To exclude the influence of blood in sockets and obtain clean images, a prototype glass probe was produced. Firstly, the glass probe and OCT were tested their ability to take images of thin bone using human dry skull. Secondly we applied them to explore implant socket that was prepared in use of crestal approach sinus lifting. As a result, real-time images of thin bone in human dry skull were acquired with the glass probe and OCT. In addition, we identified the bottom of an implant socket through the glass probe, in the case of crestal approach for sinus augmentation. However, images were not adequately acquired, when remaining teeth or surrounding bone obstructed the correct positioning of OCT apparatus. In order to expand indications, more improvements of OCT attachment should be needed.
  • Koichi Imai, Masayuki Zennyu, Yoshiaki Ono, Tsubasa Shirai
    2017 年 7 巻 1 号 p. 33-37
    発行日: 2017年
    公開日: 2020/07/18
    ジャーナル オープンアクセス
    Various studies have been conducted on regenerative medicine, by which iPS cells are differentiated to produce tissues and organs to be transplanted into the injured sites of patients. Results obtained with mouse ES cells are likely to be applied to studies on iPS and ES cell differentiation cultures. Mouse MEF (Mouse Fibroblast Feeder) cells are utilized as feeder cells. Since ES cells are derived from the reproductive organs, such as the uterus and fallopian tube, MEF cells should be applied as feeder cells to ES cell differentiation culture. Therefore, uterusand fallopian tube-derived feeder cells were prepared to examine the cardiomyocyte differentiation of ES cells. EL M3 and ES-R1-EGFP B2/EGFP cells were uterus- and fallopian tube-derived cells, respectively. They showed higher cardiomyocyte differentiation rates than the control MEF cells, whereas other fallopian tube-derived cells showed lower rates, suggesting the contamination of heterogeneous cells. Thus, the uterus- and fallopian tubederived cells also served as feeder cells, some of which were more suitable for cardiomyocyte differentiation than commercially - available feeder cells, MEF cells.
  • 本津 茂樹, 平井 瑞樹, 片山 一郎, 山本 衛, 加藤 暢宏, 吉川 一志
    2017 年 7 巻 1 号 p. 39-43
    発行日: 2017年
    公開日: 2020/07/18
    ジャーナル オープンアクセス
    審美歯科では,一般にホワイトニング法やラミネートベニア法が用いられている.しかし,これらの審美法は二度と再生することのないエナメル質に損傷を与えるという問題を抱えており,さらに後者においては接着剤によるアレルギー反応の問題もある.我々は,エナメル質の修復と審美を同時に行える新奇の審美シートの開発を試みた.まず,レーザーデポジション(PLD)法で作製した非晶質リン酸カルシウム(ACP)シートは,低密度のハイドロキシアパタイト(HAp)のターゲットを用いるほど,シート表面のパーティクルの量が増えることで白色度が高くなることがわかった.また,ACP シートをポストアニールにより結晶化したハイドロキシアパタイト(HAp)シートの白色度はシート内部に導入された粒界のためにACPシートよりさらに増加した.変色ヒト抜去歯に白色シートを重ね貼りしたときのCIE 白色度(WICIE)の変化が分光測色計により測定された.シートの重ね貼りの枚数に対応して白色度(WI)は増加し,黄色度(YI)は低下した.以上の結果より,白色HAp シートは審美シートとして非常に有望であることが分かった.
  • Patteera Sodata, Jomjai Peerapattana, Yusuke Hattori, Apa Juntavee, Ma ...
    2017 年 7 巻 1 号 p. 45-52
    発行日: 2017年
    公開日: 2020/07/18
    ジャーナル オープンアクセス
    This study aimed to simultaneously quantify the concentration of herbal and inorganic active pharmaceutical ingredients in suspensions for dental caries prevention by using near-infrared spectroscopy. Fifteen suspensions containing various concentrations of active pharmaceutical ingredients, i.e. α-mangostin and calcium phosphate containing silver ion, were prepared. The near-infrared spectrum of each suspension was measured and correlated with concentrations of active pharmaceutical ingredients by partial least square regression. The predicted results from various pre-treatments were compared to obtain the best calibration model, and was validated with the external validation data set. The equation and correlation coefficient value for the prediction of α-mangostin concentration were y = 0.8983x - 0.0184 and 0.8988, respectively. The equation and correlation coefficient value for the prediction of concentration of calcium phosphate containing silver ion were y = 0.9732x + 0.0346 and 0.9672, respectively. The results showed that near-infrared spectroscopy could predict the concentrations of both active pharmaceutical ingredients in suspensions with sufficient accuracy. It could be a useful tool for the quantitative analysis of the oral care suspensions containing the mixing of herbal and inorganic medicine.
  • Kae Ishibashi, Hisanobu Nishikawa, Kiyonori Nagata, Yusuke Hattori, Ma ...
    2017 年 7 巻 1 号 p. 53-58
    発行日: 2017年
    公開日: 2020/07/18
    ジャーナル オープンアクセス
    Orally disintegrating tablets (ODT) and films (ODF) with its rapid disintegration in the oral cavity are useful for elderly and pediatric patients to reduce choking risks. Research investigations focused on the methods and formulations of ODT and ODF has led to significant progress; however, disintegration testing methods and adequate testing devices have yet to be defined in the current Japanese Pharmacopoeia (JP17) and United State Pharmacopoeia (USP39). Hence the development of a robust and standardized method for the evaluation of disintegration and drug release properties is necessary. In the present study, the drug release mechanisms of three-layered ODF were investigated through the use of an originally developed testing system imitating the physiological conditions of the oral cavity. The drug layer was composed of hydroxypropyl cellulose (HPC) and polyethylene glycol (PEG) and was sandwiched between two coating layers composed of hydroxypropylmethyl cellulose (HPMC) and PEG. We found that the drug release of three-layered ODF was faster than that of the single drug layered ODF. We suggest that the drug release was accelerated as a result of the fast disintegration of the coating HPMC layer preceded by swelling of the drug HPC layer.
  • 照喜名 孝之, 齊藤 花江, 沼口 貴範, 服部 祐介, 大塚 誠
    2017 年 7 巻 1 号 p. 59-65
    発行日: 2017年
    公開日: 2020/07/18
    ジャーナル オープンアクセス
    Purpose Osteoporosis is a progressive and debilitating metabolic bone disease characterized by low bone mass and structural deterioration leading to increased bone fragility. Carbonated hydroxyapatite cement can be a potential biomaterial to be used as a drug carrier. We reported previously that sustainable release of simvastatin (SIM) from poly (lactic-co-glycolic acid) (PLGA) formulations could induce bone formation. The aim of this study was to develop a SIM-loaded PLGA microspheres (SPMs)/self-setting carbonated hydroxyapatite (CHAP) composite, and investigate the effect of SIM released from that composite in comparison with a SIM/CHAP composite used as a control. Methods SPMs were prepared by O/W emulsion technique. The self-setting CHAP cements were fabricated using the cement powder consisted of tetraclcium phosphate (TTCP), dicalcium phosphate dehydrate (DCPD) and NaHCO3. The SIM release from SPMs, SIM/CHAP and SPMs/CHAP cement composite scaffolds in simulated body fluid solution were determined by UV spectrometer. Cell proliferation was determined using WST-8 assay. SPMs, SIM/CHAP and SPMs/CHAP cement composite scaffolds were put into 96 well plate. MC3T3-E1 cells were seeded at 5.0 × 103 cells/well and cultured for 1, 2, 3, 4 weeks. In addition, alkaline phosphatase activity was evaluated after 7, 14, 21 days culture. Results X-ray diffraction analysis (XRD) and Fourier-transformed infrared spectroscopy (FT-IR) results showed most parts of the cement bulk powder transformed into carbonated hydroxyapatite. The SPMs and SPMs/CHAP were able to produce sustained release of SIM for 1 month, whereas the SIM/CHAP released SIM for 2 weeks. The proliferation of MC3T3-E1 cells on the SPLGAMs/CHAP showed no significant difference in comparison with the SIM/CHAP. However, the SPLGAMs/CHAP significantly increased alkaline phosphate activity, a differentiation marker of MC3T3-E1 cells, compared with the SIM/CHAP. Conclusion This study showed that the SPLGAMs/CHAP could release SIM sustainably and induce proliferation and differentiation of MC3T3-E1 more effectively than the SIM/CHAP.
  • 片山 真久寿, 玉澤 健吾, 小平 亜侑, 野浪 野浪
    2017 年 7 巻 1 号 p. 67-72
    発行日: 2017年
    公開日: 2020/07/18
    ジャーナル オープンアクセス
    The aim of this study was to develop spherical Hydroxy apatite with big diameter and porus. This material is expected to be biomatelials and protein absorbtion materials.Therefore we consider Crystal growth of few μm spherical porus hydroxy apatite (sHAp) formed with small tabular particles by simulated body fluid method in simulated body fluid We have experimented with adding sHAp1 as a seed crystal to a simulated body fluid of metastable region composition to apatite and growing it to several tens of microns or more. . When a slightly sHAp1 was added to the simulated body fluid in the metastable region, nucleation was hardly generated and the particles grew large to about 100 times, and dome-like particles were obtained. Supply amount of Ca and P to each particle increased and the particle diameter increased by decreased amount of seed crystals adding. The seed crystal growed by clystal grows tinden then supply amount of ca and p other way not spherical and dome like. Ca and P were supplied anisotropically,due to The seed crystals that grew larger by crystal growth precipitated.As the result,The seed crystal grow into dome-like shape instead of spherical shape. Hydroxy apatite particles can be grow large by the seed crystal adding into the simulated body fluid.
  • Hideo Senzaki
    2017 年 7 巻 1 号 p. 73-79
    発行日: 2017年
    公開日: 2020/07/18
    ジャーナル オープンアクセス
    The sequence of newly formed odontoblasts induced by colchicine was studied by an autoradiographic technique using 3H-proline in the rat incisor pulp. Four days after colchicine administration, marked cell proliferation and revascularization were observed in the necrotic pulp tissue. On the 6th day after colchicine administration, an organic matrix heavily labeled with 3H-prolin appeared in the cell proliferation zone and vascularity was observed adjacent to the organic matrix. The number of silver grains over the organic matrix was significantly greater than that over the other pulp tissue and that over the cell proliferation zone after 4 days. On the 8th day after colchicine administration, odontoblast-like cells were seen in the area of vascularity in contact with the organic matrix. The number of silver grains over odontoblast-like cells was significantly greater than that over the organic matrix on the 8th day or on the 6th day. On the 10th day after colchicine administration, new odontoblasts and predentine were clearly ovrerved in contact with the organic matrix. The number of silver grains over the new odontoblasts and pre-dentine was also heavily labeled with 3H-prolin. These findings suggest that new odontoblasts develop from perivascular mesenchymal stem cells during reparative dentinogenesis after colchicine destruction.
  • 尾関 和秀, 後藤 哲哉, 青木 秀希
    2017 年 7 巻 1 号 p. 81-85
    発行日: 2017年
    公開日: 2020/07/18
    ジャーナル オープンアクセス
    骨との応力適合性,審美性に優れたpolyetheretherketone(PEEK)材の骨親和性を高めるため,スパッタリング法によるHA 膜の成膜を試みた.HA 膜とPEEK 材の密着性を向上させるため,中間層にTi を用い,中間層の膜厚による透明性,細胞培養による骨親和性を評価した.成膜にはスパッタリング装置を使用し,ターゲットには,TiとHA 用い,Ti の膜厚を5 ~ 100nm,HA の膜厚を1μm とした.成膜後は,120℃で24 時間水熱処理を行い,結晶化した.骨親和性の評価には,ラット頭頂骨由来初代培養系骨芽細胞を用い,石灰化実験を行った. Ti 中間層を持つHA 膜は,PEEK 基板上へのHA 成膜が可能であった.また,Ti 中間層の膜厚が10nm 以下では膜の透明性が確認された.骨芽細胞による骨形成面積では,Ti 中間層100nm のHA 膜が最も高い骨形成面積を示し,Ti 中間層5nm のHA 膜においては,低い骨形成面積に留まった.Ti 中間層5nm のHA 膜の骨形成が不十分だった理由として,培養中にHA 膜にクラックが入ったことが一因として考えられた.
  • Satoshi Komasa, Mariko Nishizaki, Tetsuji Kusumoto, Chisato Terada, Yi ...
    2017 年 7 巻 1 号 p. 87-94
    発行日: 2017年
    公開日: 2020/07/19
    ジャーナル オープンアクセス
    Dental implants are often used to replace missing teeth, for aesthetic and practical purposes. The most common material used for dental implants is titanium, which has been used for over 40 years. New ceramic materials and composites have been developed to replace titanium-based dentures. However, these materials can suffer from discoloration, degradation, failure, and cause allergic reactions in patients. Recent studies have shown that the surface of the material plays a significant role on its stability and osseointegration. In this study, we modified the surface of a ceria-stabilized tetragonal ZrO2 polycrystalline ceramic-based nanostructured zirconia/alumina composite (NANOZR) material and titanium-based materials, and evaluated their osteoblast response including the gene expression of rat bone marrow cells on their surface. Modification of the NANOZR and titanium implant surface by alkali treatment promoted osteogenic gene expression. The increase in the osteogenic gene expression was attributed to the change in the mechanical and chemical surface properties. The findings indicate nanotopographical surface modifications can improve the osseointegration of zirconium-based implants.
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