抄録
A hybrid approach using X-ray crystallography and cryo-electron microscopy provides opportunities to reveal the activation mechanism and pathway of biological macromolecules with large conformational changes. Structural analysis of the Ca2+ release channel, inositol 1,4,5-trisphosphate receptor (IP3R) is a good example to introduce this powerful approach. Docking of the atomic coordinate of the fragment including binding domain of inositol 1,4,5-trisphosphate (IP3) and Ca2+ into the three-dimensional density map of the type 1 IP3R reconstructed from cryo-electron micrographs leads to proposing a novel regulation mechanism of Ca2+ mobilization.
