Dynamics of Redox State-Dependent Ferredoxin Structure and Electron-Transfer Complex Formation with Ferredoxin:NADP⁺ Reductase

Abstract

We have investigated the conformational and dynamical changes of plant-type ferredoxin (Fd) depending upon the redox state by 2D NMR spectroscopy and the three-dimensional structures of Fd, Fd:NADP⁺ reductase (FNR) and their complex by X-ray crystallography. These combined data allow us to discuss a molecular mechanism for the redox dependent change in the affinity of Fd with FNR, which is important in modulating redox metabolism in the cells, and also discuss the molecular property of Fd as an electron carrier in living cells.