NMR-PRE法による生体高分子複合体形成における遷移中間体の解析

抄録

Previous kinetic investigations have suggested that the process of the specific complex formation goes through intermediate states called target searches or encounter complexes, and the presence of such intermediates greatly accelerates the overall process. However, current biophysics has not provided adequate physical pictures about such intermediates. We have developed new NMR methods that permit investigations of structural and dynamics aspects of the transient, low-population intermediates in protein-DNA or protein-protein association processes. The methods make use of paramagnetic relaxation enhancement (PRE) as a tool to amplify the information about the minor species.