総説
ミトコンドリアタンパク質搬入ゲートTOM複合体の立体構造
2020 年 60 巻 5 号 p. 280-283
詳細
2020 年 60 巻 5 号 p. 280-283
The TOM complex is the main entry gate for mitochondrial proteins. Recently we determined the Cryo-EM structure of the yeast TOM complex at 3.8 Å resolution. The structure shows the dimeric form consisting of two Tom40 β-barrel channels and α-helical transmembrane subunits. The structure-based biochemical analysis revealed that presequence-containing preproteins pass through the Tom40 channel in the middle of the dimer and are transferred to the TIM23 complex. On the other hand, presequence-less preproteins leave the channel at the periphery of the TOM complex and are relayed to the chaperone proteins. Our results demonstrate the efficient transfer mechanism of preproteins.
