Stem blight of asparagus caused by Phomopsis asparagi in Myanmar and their phylogenetic grouping

抄録

In 2016, stem blight disease symptoms were occurred in asparagus fields near Pyinmana, central area of Myanmar, and samples collection was conducted. The causal fungal pathogen was isolated and the representative isolates were confirmed their pathogenicity on garden asparagus (Asparagus officinalis). The morphological characteristics indicated that all isolates produced conidiomata containing alpha condinia on potato dextrose agar (PDA) media. Six randomly selected isolates were performed on phylogenetic analysis of rDNA-ITS regions, and all isolates were in a single clade with Phomopsis asparagi (GenBank accession: KJ801806 and KJ801804). The genetic diversity of 20 isolates from Myanmar and 7 isolates from Japan were also compared by using random amplified polymorphic DNA (RAPD) analysis with eight oligo primers and repetitive-element polymerase chain reaction (rep-PCR) method with BOX and ERIC primers. In both experiment, the polymorphic banding patterns of all isolates were similar and there was almost no diversity between Myanmar and Japan isolates. This is the first characterization of P. asparagi on Asparagus in Myanmar.