A 10-kDa Fragment of Fibronectin Type III Domain Is a Neutrophil Chemoattractant Purified from Conditioned Medium of Rat Granulation Tissue

Abstract

A neutrophil chemoattractant has been purified from the conditioned medium of granulation tissue obtained from carrageenan-induced inflammation in rats. The purified chemoattractant was a basic protein with a molecular mass of 10 kDa on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) under reducing and non-reducing conditions. NH₂-terminal amino acid sequence of the purified 10-kDa protein was identical with the sequence of rat fibronectin starting from the residue Thr⁵⁸⁵, indicating that the purified 10-kDa chemoattractant is a fragment derived from the NH₂-terminal type III domain of rat fibronectin.