Biological and Pharmaceutical Bulletin
Online ISSN : 1347-5215
Print ISSN : 0918-6158
ISSN-L : 0918-6158
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Molecular Authentication of Panax ginseng Species by RAPD Analysis and PCR-RFLP
Jae-Young UMHwan-Suck CHUNGMi-Sun KIMHo-Jeong NAHyun-Jeong KWONJeong-Joong KIMKang-Min LEESeung Jae LEEJong Phil LIMKeum-Rok DOWoo-Jun HWANGYeoung-Su LYUNyeon-Hyoung ANHyung-Min KIM
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2001 Volume 24 Issue 8 Pages 872-875


In order to develop convenient and reproducible methods for the identification of ginseng drugs at a DNA level, randomly amplified polymorphic DNA (RAPD) and PCR-restriction fragment length polymorphism (PCR-RFLP) analyses were applied within Panax species. To authenticate Panax ginseng among ginseng populations, RAPD analysis was carried out using a 20 mer-random primer. The similarity coefficients among the DNA of ginseng plants analyzed were low, ranging from 0.197 to 0.491. In addition, by using PCR-RFLP analysis, very different fingerprints were obtained within Korean ginseng plants. These results suggest that these methods are able to authenticate the concerned Panax species. Broader application of this approach to authenticate other morphologically similar medicinal materials is rationalized.

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© 2001 The Pharmaceutical Society of Japan
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