Biological and Pharmaceutical Bulletin
Online ISSN : 1347-5215
Print ISSN : 0918-6158
Inhibitory Effect of Propolis on the Growth of Human Leukemia U937
Keiko AsoSyu-ichi KannoTakeshi TadanoSusumu SatohMasaaki Ishikawa
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2004 Volume 27 Issue 5 Pages 727-730


We have investigated the effect of propolis (CB Propolis) on the growth of human histiolytic lymphoma U937 cells. We found that propolis strongly inhibited the growth of the cells and macromolecular synthesis in a dose- and time-dependent manner by apoptosis. Propolis at 0.015—0.5 μl/ml showed antitumor activity with an IC50 of 0.18 μl/ml for 3 d. It also inhibits DNA, RNA and protein synthesis with an IC50 of 0.08, 0.17 and 4.3 μl/ml, respectively. The inhibitory effect on DNA synthesis was partially irreversible. Moreover, an apoptotic DNA ladder and chromatin condensation were observed in the same concentration range in which cell growth was inhibited. The caspase inhibitor, Z-Asp-CH2-DCB, prevented DNA fragmentation. These results suggest that the antitumor activity of propolis occurs through the induction of apoptosis. Propolis may be useful as a cancer chemopreventive and chemotherapeutic agent.

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© 2004 The Pharmaceutical Society of Japan
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