Biological and Pharmaceutical Bulletin
Online ISSN : 1347-5215
Print ISSN : 0918-6158
ISSN-L : 0918-6158
Notes
Inactivation of Vibrio vulnificus Hemolysin by Oligomerization but Not Proteolysis
Sung-Heui ShinHui-Yu SunMi-Hwa ChoiRa-Young ParkYoung-Hoon BaiChoon-Mee KimSoo-Young KimYoung-Ran KimShee-Eun LeeJoon-Haeng Rhee
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2005 Volume 28 Issue 7 Pages 1294-1297

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Abstract
Vibrio vulnificus extracellular protease (VvpE) is believed to destroy its hemolysin (VvhA) in the late growth phase, without obvious experimental evidence. So, we attempted to elucidate the mechanism. The hemolytic activity steeply increased with the expression of the VvhA in the early growth phase, and then abruptly declined with the expression of VvpE in the late growth phase. However, the VvhA activity also abruptly declined in a VvpE-deficient mutant. In Western blot, the degradation of VvhA was not observed; instead, the oligomerization of VvhA increased with the concomitant loss of hemolytic activity. These results evidently indicate that the inactivation of VvhA is due to the novel oligomerization of VvhA by unknown mechanism, but not to the destruction of VvhA by VvpE, so that the routine functional assay measuring hemolytic activity cannot reflect the actual production of VvhA.
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© 2005 The Pharmaceutical Society of Japan
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