Abstract
The Candida glabrata IFO 0622 strain cells obtained after cultivation at 27 °C and at 37 °C and then at 27 °C (37—27 °C) for 48 h in yeast extract-added Sabouraud liquid medium (YSLM) showed the same agglutination patterns against factor sera 1, 4, 6, and 34 in the commercially available factor serum kit ‘Candida Check’. On the other hand, the cells of the strain cultured at 37 °C had completely lost its reactivity against the factor serum 6. The enzyme-linked immunosorbent assay (ELISA) of the cell wall mannans obtained from the strain cells showed the same reactivity with the agglutination patterns against the factor sera. The 1H-nuclear magnetic resonance (NMR) pattern of the mannan obtained from the strain cells cultured at 37 °C showed that the mannan had completely lost the non-reducing β-1,2-linked mannopyranose unit in the mannotetraose Manβ1—2Manα1—2Manα1—2Man, corresponding to the serum factor 6.
