Rapid Multiplexing and Simultaneous Detection of Human Spermatogenetic Failure with a 12 Lane Microchip Electrophoresis System

Mohammad Jabasini; Ashraf Abdulazim Ewis; Maged Fouad; Fuquan Dang; Guichen Ping; Toshikatsu Shinka; Yutaka Nakahori; Noritada Kaji; Manabu Tokeshi; Yoshinobu Baba

doi:10.1248/bpb.29.1487

Notes

Rapid Multiplexing and Simultaneous Detection of Human Spermatogenetic Failure with a 12 Lane Microchip Electrophoresis System

Mohammad Jabasini, Ashraf Abdulazim Ewis, Maged Fouad, Fuquan Dang, Guichen Ping, Toshikatsu Shinka, Yutaka Nakahori, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba

Author information

Mohammad Jabasini
Department of Applied Chemistry, Graduate School of Engineering, Nagoya University
Toray Industries, Inc., New Frontiers Research Laboratories
Ashraf Abdulazim Ewis
Nanobio Research Team, Health Technology Research Center, National Institute of Advanced Industrial Science and Technology, Japan
Maged Fouad
Department of Applied Chemistry, Graduate School of Engineering, Nagoya University
Fuquan Dang
Nanobio Research Team, Health Technology Research Center, National Institute of Advanced Industrial Science and Technology, Japan
Guichen Ping
Department of Applied Chemistry, Graduate School of Engineering, Nagoya University
Toshikatsu Shinka
Department of Human Genetics and Public Health, School of Medicine, The University of Tokushima
Yutaka Nakahori
Department of Human Genetics and Public Health, School of Medicine, The University of Tokushima
Noritada Kaji
Department of Applied Chemistry, Graduate School of Engineering, Nagoya University
Manabu Tokeshi
Department of Applied Chemistry, Graduate School of Engineering, Nagoya University
Yoshinobu Baba
Department of Applied Chemistry, Graduate School of Engineering, Nagoya University
Nanobio Research Team, Health Technology Research Center, National Institute of Advanced Industrial Science and Technology, Japan

Keywords: spermatogenic failure, multiplex PCR, 12-lane multichannel microchip

JOURNAL FREE ACCESS

2006 Volume 29 Issue 7 Pages 1487-1489

DOI https://doi.org/10.1248/bpb.29.1487

Details

Abstract

For the amplification and ultrafast separation of the genetic markers and DNA sequences that are related to human male infertility, a multiplex PCR for amplifying three DNA sequence-tagged sites (STS) located on the human Y chromosome with possible roles in the spermatogenesis process has been designed and applied followed by separation on a microchip. First, the optimum T_m degree for the three DNA markers was optimized and determined experimentally, and the three DNA STS were amplified. These three DNA markers were then separated on a 12-lane microchip electrophoresis system, which can analyze the DNA markers on 12 channels simultaneously. The combination of these two technologies, multiplex PCR and microchip electrophoresis, allows the analysis of 36 DNA markers (12×3) within only 180 s.

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