Biological and Pharmaceutical Bulletin
Online ISSN : 1347-5215
Print ISSN : 0918-6158
Genotoxic and Cytotoxic Effects Produced by Acetogenins Obtained from Annona cherimolia MILL.
Karol Karla García-AguirreLuis Gerardo Zepeda-VallejoEva Ramón-GallegosIsela Alvárez-GonzálezEduardo Madrigal-Bujaidar
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Volume 31 (2008) Issue 12 Pages 2346-2349

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In the present study we extracted three isomeric acetogenins (Ace) from the seeds of Annona cherimolia MILL. (Annonaceae) and determined their genotoxic and cytotoxic potential in mice. Our results showed a significant increase in the rate of micronucleated polychromatic erythrocytes induced by Ace with respect to the value for the control group; the effect was less pronounced than that observed with daunorubicin (Dau). To evaluate cytotoxicity, we determined the proportion of polychromatic erythrocytes with respect to the number of normochromatic erythrocytes, and we found an inhibitory effect induced by Ace that was quite similar to that observed with Dau. Besides, by means of the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide test, we also determined the cytotoxicity of Ace in both a normal fibroblast mouse cell line and in a line derived from human colon cancer. In this assay, the strongest decrease in viability corresponded to the malignant cell line. Our results established for the first time the genotoxic capacity of these compounds in vivo, and confirmed their cytotoxic potential in cultured cells.

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© 2008 The Pharmaceutical Society of Japan
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