Biological and Pharmaceutical Bulletin
Online ISSN : 1347-5215
Print ISSN : 0918-6158
Molecular Target of Piperine in the Inhibition of Lipid Droplet Accumulation in Macrophages
Daisuke MatsudaSatoshi OhteTaichi OhshiroWei JiangLawrence RudelBin HongShuyi SiHiroshi Tomoda
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2008 Volume 31 Issue 6 Pages 1063-1066


An alkaloid piperine isolated from the Piper Nigrum was found to inhibit lipid droplet accumulation in mouse macrophages, and especially inhibited cholesteryl ester (CE) synthesis (IC50: 25 μM). The metabolism of cholesterol from lysosome to lipid droplet was inhibited with a similar IC50 (18 μM), indicating that the site of inhibition is one of the steps between the lysosomes and the endoplasmic reticulum. Therefore, effects of piperine on acyl-CoA:cholesterol acyltransferase (ACAT) activity in the microsomes prepared from mouse macrophage and liver were studied, to show that the compounds inhibited the activity in both cases (IC50: 9.1, 7.0 μM, respectively). Furthermore, piperine was found to inhibit both ACAT1 and ACAT2 isozymes to a similar extent (IC50: 16, 18 μM, respectively) in cell-based assays using ACAT1- or ACAT2-expressing cells. Thus, it was suggested that piperine inhibited macrophage ACAT to decrease CE synthesis, leading to a reduction of lipid droplets.

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© 2008 The Pharmaceutical Society of Japan
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