抄録
We measured the activity and stability of three mannosyltransferases to ascertain the mechanisms of changes in the antigenicity and the mannan structure of Candida albicans cells cultured at high temperature (37 °C) under acidic (pH 2.0) conditions in a liquid medium. The α-1,6-mannosyltransferase (α-1,6-MT) activity of the particulate-insoluble enzyme fractions prepared from C. albicans J-1012 (J-1012) cells cultured at 37 °C was retained compared to those at 27 °C, whereas β-1,2-mannosyltransferase II (β-1,2-MT II) activity was detected in the 27 °C fraction but not in the 37 °C fraction. Similar results were obtained in the fraction prepared from J-1012 cells cultured at pH 2.0. The α-1,6-MT activities of fractions prepared from C. albicans NIH B-792 (B-792) strain cells cultured at 37 °C were retained compared to those at 27 °C, whereas β-1,2-mannosyltransferase VI-6 (β-1,2-MT VI-6) activity was detected in the fraction of B-792 cells cultured at 27 °C but not detected in the 37 °C fraction. We also found that the β-1,2-MT II and β-1,2-MT VI-6 activity of C. albicans cells was more sensitive to both high temperature and low pH compared with α-1,6-MT activity.
