Biological and Pharmaceutical Bulletin
Online ISSN : 1347-5215
Print ISSN : 0918-6158
ISSN-L : 0918-6158
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267件中1~20件の論文を表示しています
  • 47 巻 (2024) 1 号 p. 245-252
    Rheological Properties and Composition Affecting the Skin Permeation of a Model of a Hydrophilic Drug in Lecithin Reverse Wormlike Micelles もっと読む
  • 47 巻 (2024) 1 号 p. 104-111
    Ethoxyquin, a Lipid Peroxidation Inhibitor, Has Protective Effects against White Matter Lesions in a Mouse Model of Chronic Cerebral Hypoperfusion もっと読む
  • 47 巻 (2024) 1 号 p. 72-78
    Magnitude of Fruit Juice–Drug Interactions Due to Osmolality-Dependent Fluid Secretion: Differences among Apple, Orange, and Grapefruit Juices もっと読む
  • 47 巻 (2024) 1 号 p. 60-71
    Involvement of Cannabinoid Receptors and Adenosine A2B Receptor in Enhanced Migration of Lung Cancer A549 Cells Induced by γ-Ray Irradiation もっと読む
  • 47 巻 (2024) 1 号 p. 28-36
    Identification of Azalamellarin N as a Pyroptosis Inhibitor もっと読む
  • 46 巻 (2023) 12 号 p. 1661-1665
    Framework-Directed Amino-Acid Insertions Generated over 55-Fold Affinity-Matured Antibody Fragments That Enabled Sensitive Luminescent Immunoassays of Cortisol もっと読む
    編集者のコメント

    Genetic engineering now enables generation of artificially modified antibodies having higher diagnostic utilities. The authors developed single-chain Fv fragments (scFvs) against cortisol with >55-fold improved affinity (Ka, 2.0-2.2 ´ 1010 M-1) by inserting additional amino acid(s) site-directedly into the framework region 1 of the VH domain. These scFvs were fused with NanoLuc luciferase for the use in an enzyme-linked immunosorbent assay (ELISA) system. The resulting luminescent ELISAs generated dose-response curves with >150-fold higher sensitivity than the colorimetric ELISAs using the scFv without insertion and >8,000-fold higher sensitivity than the ELISA using the mouse antibody from which the scFvs were derived.

  • 46 巻 (2023) 12 号 p. 1676-1682
    Method for Preparing Recombinant Galectin-2 Protein without Escherichia coli-Specific Post-translational Modifications もっと読む
    編集者のコメント

    E. coli is often employed for the cost-effective production of large quantities of recombinant proteins. Conventionally, it is believed that post-translational modifications, including glycosylation, do not transpire during protein expression in E. coli. However, in the course of preparing recombinant galectin-2 protein using E. coli, the authors discovered that phosphogluconoylation of Lys residues and mistranslation of termination codons occurred. The authors have elucidated strategies to mitigate these occurrences, proposing the addition of tags, substitution of Lys residues, and modification of termination codons. These methods offer valuable means to prevent undesired modifications, ensuring the production of homogeneous recombinant proteins in E. coli.

  • 46 巻 (2023) 12 号 p. 1720-1730
    Exploring Cell-Penetrating Peptides as Penetration Enhancers in Eye Drop Formulations Using a Reconstructed Human Corneal Epithelial Model もっと読む
    編集者のコメント

    The authors focused on cell-penetrating peptides (CPPs) as penetration enhancers for ocular drug delivery. This study suggested that the CPPs evaluated in this study can be penetration enhancers based on in vitro intracellular uptake using a reconstructed human corneal epithelial model. The CPPs could enhance the penetration of drug molecules into the cornea in cases of coexistence as well as conjugation between CPPs and drug molecules. The result of surface plasmon resonance showed that the electrostatic interaction plays an important role. The authors expect that this fundamental information in this article will support the development of new penetration enhancers in eye drop formulations for ocular drug delivery.

  • 46 巻 (2023) 12 号 p. 1753-1760
    Arid5a/IL-6/PAI-1 Signaling Is Involved in the Pathogenesis of Lipopolysaccharide-Induced Kidney Injury もっと読む
    編集者のコメント

    Inflammation is responsible for the development of various kidney diseases. Plasminogen activator inhibitor-1 (PAI-1) is involved in the pathogenesis of inflammatory kidney injury; however, the regulatory mechanism of PAI-1 in injured kidneys remains unclear. The authors found that PAI-1 expression was increased in endothelial cells after lipopolysaccharide (LPS, an inflammation inducer) treatment, and pharmacological inhibition of PAI-1 reduced LPS-induced kidney injury. Moreover, IL-6 exacerbated kidney injury concomitant with increased PAI-1 expression, and Arid5a deficiency partially suppressed the expression of IL-6 and PAI-1 in the kidneys after LPS treatment. These findings indicate that the Arid5a/IL-6/PAI-1 signaling is involved in LPS-induced kidney injury.

  • 46 巻 (2023) 12 号 p. 1778-1786
    Identification of the Acidification Mechanism of the Optimal pH for RNase He1 もっと読む
    編集者のコメント

    Hericium erinaceus secretes an acidic ribonuclease (RNase) He1 belonged to RNase T1 family. The authors decided on the structure of He1 apo form and He1/guanosine complex. The mechanism of acidification of optimal pH in He1 was, in neutral environment, to form the hydrogen bond between Asp 31 on α1β3- loop and His 34 (catalytic residue), and repulsive each other Glu 92 and Asp 93 on β6,7- loop. Structure comparison of He1 with other acidic RNases, Ms and U2, suggested that the acidic residues on α1β3- and β6,7- loop may contribute to the acidification of optimal pH in Ms and U2.

  • 46 巻 (2023) 11 号 p. 1498-1505
    Suppression of Lipopolysaccharide-Induced IL-1β Gene Expression by High-Molecular-Weight Adiponectin in RAW264.7 Macrophages もっと読む
    編集者のコメント

    Although most previous studies in this area used recombinant adiponectin, herein, the author used native high-molecular-weight (HMW) adiponectin purified from human plasma, which is considered the most active form of circulating adiponectin. The current results clearly demonstrate that native HMW adiponectin preferentially inhibits lipopolysaccharide-induced interleukin-1β expression but not tumor necrosis factor-α expression by inhibiting the Akt-C/EBPβ inflammatory signaling pathway in macrophages. Furthermore, HMW adiponectin preconditioning is essential for achieving the anti-inflammatory effects of adiponectin. Thus, these findings highlight the regulatory mechanisms underlying the anti-inflammatory function of adiponectin.

  • 46 巻 (2023) 11 号 p. 1535-1547
    Turkish Plants, Including Quercetin and Oenothein B, Inhibit the HIV-1 Release and Accelerate Cell Apoptosis もっと読む
    編集者のコメント

    Authors suggest that plant extracts, including quercetin and oenothein B, reduce the amount of virus in the cell supernatants and induce cytotoxicity in HIV-1-infected T cells but not HTLV-1-infected T cells. The large amount of oenothein B were detected in Onagraceae. Thus, the plant extracts might block the HIV-1 release and kill the HIV-1-infected cells. Consequently, the plant extracts from the plant library of Turkey might be suitable candidates to develop novel anti-retroviral drugs that target the late phase of the HIV-1 life cycle.

  • 46 巻 (2023) 11 号 p. 1583-1591
    Endothelium-Independent Vasorelaxant Effects of Sudachitin and Demethoxysudachitin, Polymethoxyflavone from the Peel of Citrus sudachi on Isolated Rat Aorta もっと読む
    編集者のコメント

    Citrus sudachi is a popular fruit in Tokushima Prefecture, Japan, and its peel contains high amounts of polymethoxyflavones with the most abundant being sudachitin (SDC) followed by demethoxysudachitin (DMSDC). The effects of SDC and DMSDC on the cardiovascular system have not been investigated. The present study aimed to investigate the effects of SDC and DMSDC on vascular tonus and to investigate mechanism of action of SDC using aorta preparations isolated from rats. The results demonstrated that SDC and DMSDC cause endothelial-independent relaxation, and that the mechanism of vasorelaxation by SDC is associated with the enhancement of cAMP- and cGMP-dependent pathways.

  • 46 巻 (2023) 11 号 p. 1601-1608
    Differential Contribution of 5-HT4, 5-HT5, and 5-HT6 Receptors to Acute Pruriceptive Processing Induced by Chloroquine and Histamine in Mice もっと読む
    編集者のコメント

    Antidepressants, such as milnacipran, a serotonin and noradrenaline reuptake inhibitor, and mirtazapine, a noradrenergic and specific serotonergic antidepressant attenuate the induction of scratching events by chloroquine (CQ) or histamine. However, it remains unclear whether serotonin or noradrenaline is involved in attenuating effects of these antidepressants. Miyahara and colleagues show that 5-HT4 and 5-HT6 receptors are involved in the amelioration of CQ-induced scratches, but not histamine-induced scratches, engendered by the antidepressants. These findings suggest that 5-HT4, 5-HT5, and 5-HT6 receptors play differential roles in acute pruriceptive processing after administration of CQ or histamine.

  • 46 巻 (2023) 11 号 p. 1609-1618
    Effects of Body Composition on Renal Function Estimates in Older Patients もっと読む
    編集者のコメント

    The authors validated the modified Cockcroft-Gault equation, developed previously for aged-oriented cohort, in a newly obtained dataset and found that good renal function estimates were obtained for patients exceeding 65 years of age. Using statistical analysis, estimates for a subset of patients in this cohort were identified to be inadequate and this deviation from estimates was attributed to a decreased albumin level. A multivariate linear regression estimating equation was developed for this region by incorporating body composition parameters. A flow diagram was proposed to select an appropriate renal function estimating equation particularly for older patients.

  • 46 巻 (2023) 10 号 p. 1365-1370
    Comparison of Incidence of Hyponatremia between Linezolid and Vancomycin by Propensity Score Matching Analysis もっと読む
    編集者のコメント

    Linezolid (LZD) has been reported to cause severe hyponatremia, but infection per se is also a potential cause of hyponatremia by increasing vasopressin secretion. The author compared the incidence and risk of developing hyponatremia between LZD and vancomycin (VCM) using propensity score analysis to demonstrate that hyponatremia is associated with LZD rather than infection. The analysis indicated a significantly higher incidence and 3.7-fold higher risk of developing hyponatremia associated with LZD than with VCM, regardless of patient background characteristics. Regularly monitoring serum sodium and infusing sodium promptly when the level decreases would be required when administering LZD.

  • 46 巻 (2023) 10 号 p. 1421-1426
    Therapeutic Effects of Albumin-Fused BMP7 on 2 Experimental Models of Liver Fibrosis もっと読む
    編集者のコメント

    Despite the fact that liver fibrosis is an intractable disease with a poor prognosis, effective therapeutic agents are not available. The authors focused on bone morphogenetic factor 7 (BMP7) that inhibits TGF-β signaling. They prepared a long-acting albumin-fused BMP7 (HSA-BMP7) and evaluated its inhibitory effect on liver fibrosis. In the mice with bile duct ligation, HSA-BMP7 administration suppressed the infiltration of inflammatory cells and fibrosis area around the bile duct. In the carbon tetrachloride-induced liver fibrosis mice, HSA-BMP7 administration also decreased the hepatic fibrosis. HSA-BMP7 has the potential for serving as a therapeutic agent for the treatment of liver fibrosis.

  • 46 巻 (2023) 10 号 p. 1427-1434
    Lymphatic Transport and Immune Activation Effect by Locally Administered Extracellular Vesicles from Saccharomyces cerevisiae as Biocompatible Vaccine Adjuvants もっと読む
    編集者のコメント

    The yeast strain Saccharomyces cerevisiae is an eukaryotic organism that secrete extracellular vesicles (EVs). The authors characterized the biodistribution of locally (intradermally and subcutaneously) administered Saccharomyces cerevisiae-derived EVs (S-EVs) and the EV-mediated immune responses to evaluate their potential use as biocompatible vaccine adjuvants. Locally administered S-EVs were delivered to the lymph nodes, mainly reaching the B-cell zone. Furthermore, S-EVs increased the expression of cytokine and costimulatory molecules. Especially, subcutaneously injected S-EVs showed potent adjuvanticity, indicating that subcutaneous administration of S-EVs is the desirable approach for achieving effective immune stimulation. These findings will facilitate the development of novel EV-based immunotherapies.

  • 46 巻 (2023) 10 号 p. 1435-1443
    Osteogenic Effects of KY-054, a Novel Coumarin Derivative on Femur Cortical Bone in Ovariectomized Female Rats もっと読む
    編集者のコメント

    Osteoporosis is caused by an imbalance between osteoblast bone formation and osteoclast bone resorption, thus is treated with oral and parenteral bone-resorption inhibitors and parenteral bone-formation promoting drugs. The authors demonstrated that KY-054, a novel coumarin derivative, has osteoblast differentiation-promoting activities in mouse and rat mesenchymal stem cells, increasing metaphyseal and diaphyseal cortical bone volume and bone strength parameters without reducing medullary volume by micro-computed tomography in ovariectomized rats. KY-054 is a potential orally active osteogenic anti-osteoporosis drug candidate with a unique mode of action by acceleration of osteoblast differentiation.

  • 46 巻 (2023) 10 号 p. 1461-1467
    Fabrication of 3D-Printed Contact Lens Composed of Polyethylene Glycol Diacrylate for Controlled Release of Azithromycin もっと読む
    編集者のコメント

    Authors fabricated 3D-printed contact lenses composed of polyethylene glycol diacrylate and an antibiotic, azithromycin. The drug-loaded contact lenses were prepared using vat photopolymerization 3D printer, and the effect of second curing on 3D printed object was investigated. The mechanical strength and drug release of contact lens formulation were evaluated in the present study. The 3D printed contact lenses exhibited antimicrobial effect in vitro. The current results suggested that the preparation of antibiotic-loaded contact lenses by 3D printing provides useful information for manufacture of ophthalmic device and personalized therapy.

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