Biological and Pharmaceutical Bulletin
Online ISSN : 1347-5215
Print ISSN : 0918-6158
ISSN-L : 0918-6158
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Expression of Pkd2l2 in Testis Is Implicated in Spermatogenesis
Ye ChenZheng ZhangXiao-Yan LvYi-Dong WangZhong-Guo HuHuan SunRui-Zhi TanYu-Hang LiuGuo-Hui BianYan XiaoQin-Wei LiQiu-Tan YangJian-Zhong AiLu FengYang YangYu-Quan WeiQin Zhou
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2008 Volume 31 Issue 8 Pages 1496-1500


Pkd2l2 is a novel member of the polycystic kidney disease (PKD) gene family in mammals. Prominently expressed in testis, this gene is still poorly understood. In this study, reverse transcription polymerase chain reaction (RT-PCR) results showed a time-dependent expression pattern of Pkd2l2 in postnatal mouse testis. Immunohistochemical analysis revealed that Pkd2l2 encoded a protein, polycystin-L2, which was predominantly detectable in the plasma membrane of spermatocytes and round spermatids, as well as in the head and tail of elongating spermatids within seminiferous tubules in mouse testis tissue sections of postnatal day 14 and adult mice. A green fluorescent fusion protein of Pkd2l2 resided in the plasma membrane of HEK 293 and MDCK cells, suggesting that it functions as a plasma membrane protein. Overexpression of Pkd2l2 increased the intracellular calcium concentration of MDCK cells, as detected by flow cytometry. Collectively, these data indicated that Pkd2l2 may be involved in the mid-late stage of spermatogenesis through modulation of the intracellular calcium concentration.

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© 2008 The Pharmaceutical Society of Japan
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