Biological and Pharmaceutical Bulletin
Online ISSN : 1347-5215
Print ISSN : 0918-6158
ISSN-L : 0918-6158
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Clitocybins, Novel Isoindolinone Free Radical Scavengers, from Mushroom Clitocybe aurantiaca Inhibit Apoptotic Cell Death and Cellular Senescence
Eun-Yi MoonJin-Mi OhYoung-Hee KimIn-Ja RyooIck-Dong Yoo
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2009 Volume 32 Issue 10 Pages 1689-1694

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Abstract

High concentration of intracellular reactive oxygen species (ROS) plays a role in damaging biological systems. We isolated clitocybin A from the culture broth of Clitocybe aurantiaca and then clitocybin B and C derivatives were synthesized from clitocybin A. IMR-90 lung fibroblast cells were pre-treated or post-treated with clitocybin A, B and C to the addition of 100 μM H2O2. These compounds inhibited the level of intracellular reactive oxygen species (ROS) and H2O2-induced cell death as judged by hypodiploid cell formation. The inhibitory effect of clitocybins on H2O2-induced cell death was comparable to that with N-acetylcysteine (NAC), a well-known ROS scavenger. The inhibition of H2O2-induced cell death by clitocybins was mediated by the reduction of caspase 3 and 9 activation, cytochrome c release from mitochondria and the degradation of IκB-α and IκB-β, which could be resulted in the prevention of cellular senescence. It suggests that clitocybins are novel compounds scavenging ROS and protect cells from apoptosis and cellular senescence.

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© 2009 The Pharmaceutical Society of Japan
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