Biological and Pharmaceutical Bulletin
Online ISSN : 1347-5215
Print ISSN : 0918-6158
ISSN-L : 0918-6158
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Utility Decay Rates of T1-Weighted Magnetic Resonance Imaging Contrast Based on Redox-Sensitive Paramagnetic Nitroxyl Contrast Agents
Ken-ichiro Matsumoto
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2009 Volume 32 Issue 4 Pages 711-716

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Abstract

The availability and applicability of the combination of paramagnetic nitroxyl contrast agent and T1-weighted gradient echo (GE)-based dynamic magnetic resonance imaging (MRI) measurement for redox imaging are described. The time courses of T1-weighted GE MRI signal intensities according to first-order paramagnetic loss of a nitroxyl contrast agent were simulated for several experimental conditions. The apparent decay rate calculated based on decreasing T1-weighted MRI contrast (kMRI) can show an approximate value of the original decay rate (ktrue) discretionarily given for simulation with suitable experimental parameters. The difference between kMRI and ktrue can be sufficiently small under T1-weighted spoiled gradient echo (SPGR) scan conditions (repetition time=75 ms, echo time=3 ms, and flip angle=45°), with a conventional redox-sensitive nitroxyl contrast agent, such as 4-hydroxy-2,2,6,6,-tetramethylpiperidine-N-oxyl (TEMPOL) and/or 3-carbamoyl-2,2,5,5-tetramethylpyrrolidine-N-oxyl (carbamoyl-PROXYL), and with i.v. doses of below 1.5 μmol/g b.w. for mice. The results of this simulation suggest that the kMRI of nitroxyl contrast agents can be the primary index of redox status under biological conditions.

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© 2009 The Pharmaceutical Society of Japan
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