Development of Randomly Amplified Polymorphic DNA-Sequence Characterized Amplified Region Marker for Identification of Apocynum venetum L<small>INN.</small> from A. pictum S<small>CHRENK</small>

Changmei Lu; Weiming Zhang; Xuemei Peng; Gongping Gu; Minmin Chen; Zezi Tang

doi:10.1248/bpb.33.522

Notes

Development of Randomly Amplified Polymorphic DNA-Sequence Characterized Amplified Region Marker for Identification of Apocynum venetum LINN. from A. pictum SCHRENK

Changmei Lu, Weiming Zhang, Xuemei Peng, Gongping Gu, Minmin Chen, Zezi Tang

著者情報

キーワード: Apocynum venetum, Apocynum pictum, identification, sequence characterized amplified region, randomly amplified polymorphic DNA, bulked segregate analysis

ジャーナルフリー

2010 年 33 巻 3 号 p. 522-526

DOI https://doi.org/10.1248/bpb.33.522

詳細

抄録

Apocynum venetum LINN. is an important Chinese crude drug, and its sibling species A. pictum SCHRENK is a confusable herb which is similar to it. The purpose of this study is to develop DNA molecular markers to distinguish A. venetum from A. pictum through the combinative technologies of bulked segregate analysis (BSA) and randomly amplified polymorphic DNA (RAPD). Two putative markers B08-407 and B03-1368 specific for A. venetum were identified and sequenced. Based on the sequence information, two pairs of primers were designed and synthesized for sequence characterized amplified region (SCAR) markers. But only one primer pair, B03-1368, produced a clear SCAR band in all samples of A. venetum and not in A. pictum. This SCAR marker was found useful for rapid identification of A. venetum from A. pictum.

責任著者(Corresponding author)

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