Identification and Detection Method for Genetically Modified Papaya Resistant to Papaya Ringspot Virus YK Strain

Kosuke Nakamura; Hiroshi Akiyama; Kiyomi Ohmori; Yuki Takahashi; Reona Takabatake; Kazumi Kitta; Hiroyuki Nakazawa; Kazunari Kondo; Reiko Teshima

doi:10.1248/bpb.34.1648

Communication to the Editor

Identification and Detection Method for Genetically Modified Papaya Resistant to Papaya Ringspot Virus YK Strain

Kosuke Nakamura, Hiroshi Akiyama, Kiyomi Ohmori, Yuki Takahashi, Reona Takabatake, Kazumi Kitta, Hiroyuki Nakazawa, Kazunari Kondo, Reiko Teshima

Author information

Keywords: genetically modified organism, papaya, polymerase chain reaction, genomic DNA

JOURNAL FREE ACCESS

2011 Volume 34 Issue 10 Pages 1648-1651

DOI https://doi.org/10.1248/bpb.34.1648

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Abstract

Unauthorized genetically modified (GM) papaya (Carica papaya LINNAEUS) was detected in a commercially processed product, which included papaya as a major ingredient, in Japan. We identified the transgenic vector construct generated based on resistance to infection with the papaya ringspot virus (PRSV) YK strain. A specific detection method to qualitatively monitor papaya products for contamination with the GM papaya was developed using the real-time polymerase chain reaction.

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