Biological and Pharmaceutical Bulletin
Online ISSN : 1347-5215
Print ISSN : 0918-6158
Notes
Caffeic Acid Phenethyl Ester Inhibits Endothelial Tissue Factor Expression
Cathérine GebhardBarbara Elisabeth StähliStephanie LargiadèrErik Walter HolyAlexander AkhmedovGiovanni Guido CamiciThomas Felix LüscherFelix Christoph Tanner
Author information
JOURNALS FREE ACCESS FULL-TEXT HTML

2013 Volume 36 Issue 6 Pages 1032-1035

Details
Abstract

Caffeic acid phenethyl ester (CAPE) is a component of honeybee hives with various beneficial properties. Tissue factor (TF), the key trigger of thrombosis, is expressed in human endothelial cells. This study was designed to investigate whether CAPE modulates TF expression in human aortic endothelial cells (HAECs). Western blots and real-time polymerase chain reactions were performed. CAPE (10−7–10−5m) inhibited tumor necrosis factor (TNF)-α induced endothelial TF protein expression by 2.1-fold at 10−5m (p<0.0001). Similarly, TF surface activity was reduced (p<0.02). In contrast, TF mRNA expression, TF promoter activity, and mitogen-activated protein (MAP) kinase activation remained unaltered. In conclusion, CAPE inhibits TF protein expression and activity at the posttranscriptional level thereby exhibiting anti-thrombotic potential.

Information related to the author
© 2013 The Pharmaceutical Society of Japan
Previous article Next article
feedback
Top