Growth Inhibition of an Opportunistic Yeast Pathogen Trichosporon asahii by Staphylococcus epidermidis

Reiko Ikeda; Yuki Ogasawara; Kazuhiko Takatori; Tomoe Ichikawa; Miki Nakajima; Kazuko Harigaya; Miho Watanabe; Erika Okudaira; Hanari Yoshikawa; Kazuki Yanagisawa

doi:10.1248/bpb.b16-01000

Regular Articles

Growth Inhibition of an Opportunistic Yeast Pathogen Trichosporon asahii by Staphylococcus epidermidis

Reiko Ikeda , Yuki Ogasawara, Kazuhiko Takatori, Tomoe Ichikawa, Miki Nakajima, Kazuko Harigaya, Miho Watanabe, Erika Okudaira, Hanari Yoshikawa, Kazuki Yanagisawa

Author information

Reiko Ikeda Corresponding author
Department of Microbial Science and Host Defense, Meiji Pharmaceutical University
Yuki Ogasawara
Department of Analytical Biochemistry, Meiji Pharmaceutical University
Kazuhiko Takatori
Graduate School of Pharmaceutical Sciences, Meiji Pharmaceutical University
Tomoe Ichikawa
Department of Microbial Science and Host Defense, Meiji Pharmaceutical University
Miki Nakajima
Department of Microbial Science and Host Defense, Meiji Pharmaceutical University
Kazuko Harigaya
Department of Microbial Science and Host Defense, Meiji Pharmaceutical University
Miho Watanabe
Department of Microbial Science and Host Defense, Meiji Pharmaceutical University
Erika Okudaira
Department of Microbial Science and Host Defense, Meiji Pharmaceutical University
Hanari Yoshikawa
Department of Microbial Science and Host Defense, Meiji Pharmaceutical University
Kazuki Yanagisawa
Department of Microbial Science and Host Defense, Meiji Pharmaceutical University

Keywords: Trichosporon asahii, Staphylococcus epidermidis, lactic acid, electron ionization-high-resolution mass spectrometry

JOURNAL FREE ACCESS FULL-TEXT HTML

2017 Volume 40 Issue 5 Pages 693-697

DOI https://doi.org/10.1248/bpb.b16-01000

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Abstract

In the co-culture of Staphylococcus epidermidis and Trichosporon asahii, a fungal pathogen, it was observed that live S. epidermidis inhibited the growth of T. asahii. Soluble active anti-T. asahii substances were speculated to be produced by S. epidermidis in culture medium. Using ¹H- and ¹³C-NMR spectra and electron ionization-high resolution mass spectrometry (HR-negative-FAB-MS), we separated the active molecule and identified it as lactic acid. Commercially available L-lactic acid and D-lactic acid inhibited the growth of T. asahii. These results show that metabolites from bacterial populations are involved in the interactions of pathogenic fungi. The use of antibacterial agents to treat primary diseases could lead to the disruption of normal microbial communities and could cause opportunistic infections such as trichosporonosis.

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