Measurement of the Estradiol Concentration in Cerebrospinal Fluid from Infants and Its Correlation with Serum Estradiol and Exosomal MicroRNA-126-5p

Masahiro Ishii; Ayako Senju; Ami Oguro; Masayuki Shimono; Shunsuke Araki; Koichi Kusuhara; Kouichi Itoh; Mayumi Tsuji; Yasuhiro Ishihara

doi:10.1248/bpb.b20-00549

Notes

Measurement of the Estradiol Concentration in Cerebrospinal Fluid from Infants and Its Correlation with Serum Estradiol and Exosomal MicroRNA-126-5p

Masahiro Ishii, Ayako Senju, Ami Oguro, Masayuki Shimono, Shunsuke Araki, Koichi Kusuhara, Kouichi Itoh, Mayumi Tsuji, Yasuhiro Ishihara

Author information

Masahiro Ishii
Department of Pediatrics, School of Medicine, University of Occupational and Environmental Health
Ayako Senju
Department of Pediatrics, School of Medicine, University of Occupational and Environmental Health
Ami Oguro
Program of Biomedical Science, Graduate School of Integrated Sciences for Life, Hiroshima University
Masayuki Shimono
Department of Pediatrics, School of Medicine, University of Occupational and Environmental Health
Shunsuke Araki
Department of Pediatrics, School of Medicine, University of Occupational and Environmental Health
Koichi Kusuhara
Department of Pediatrics, School of Medicine, University of Occupational and Environmental Health
Kouichi Itoh
Laboratory for Pharmacotherapy and Experimental Neurology, Kagawa School of Pharmaceutical Sciences, Tokushima Bunri University
Mayumi Tsuji
Department of Environmental Health, School of Medicine, University of Occupational and Environmental Health
Yasuhiro Ishihara Corresponding author
Program of Biomedical Science, Graduate School of Integrated Sciences for Life, Hiroshima University

Keywords: estradiol, cerebrospinal fluid, microRNA, infant

JOURNAL FREE ACCESS FULL-TEXT HTML

2020 Volume 43 Issue 12 Pages 1966-1968

DOI https://doi.org/10.1248/bpb.b20-00549

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Abstract

Estradiol has an important role in the brain, such as in neuronal development and protection, but estradiol levels in the human brain have not been well investigated. In this study, we measured the estradiol concentration in the cerebrospinal fluid (CSF) of infants to reveal the relationships between the estradiol concentrations in the serum and the CSF and further determined exosomal microRNAs in serum. Estradiol in the CSF was strongly correlated with serum estradiol and moderately correlated with miR-126-5p in the serum exosomes. This report is the first to determine the estradiol concentration in CSF from infants and showed that the levels of miR-126-5p as well as serum estradiol can be candidates to predict brain estrogen status.

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