Identification and Detection of Genetically Modified Papaya Resistant to Papaya Ringspot Virus Strains in Thailand

Kosuke Nakamura; Kazunari Kondo; Tomoko Kobayashi; Akio Noguchi; Kiyomi Ohmori; Reona Takabatake; Kazumi Kitta; Hiroshi Akiyama; Reiko Teshima; Tomoko Nishimaki-Mogami

doi:10.1248/bpb.b13-00721

This article has now been updated. Please use the final version.

Identification and Detection of Genetically Modified Papaya Resistant to Papaya Ringspot Virus Strains in Thailand

Kosuke Nakamura, Kazunari Kondo, Tomoko Kobayashi, Akio Noguchi, Kiyomi Ohmori, Reona Takabatake, Kazumi Kitta, Hiroshi Akiyama, Reiko Teshima, Tomoko Nishimaki-Mogami

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Keywords: genetically modified organism, papaya, polymerase chain reaction, genomic DNA

JOURNAL FREE ACCESS Advance online publication

Article ID: b13-00721

DOI https://doi.org/10.1248/bpb.b13-00721

The final version of this article is now available: Vol. 37 (2014), No. 1 pp. 1-5

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Abstract

Many lines of genetically modified (GM) papaya (Carica papaya Linnaeus) have been developed worldwide to resist infection from various strains of papaya ringspot virus (PRSV). We found an unidentified and unauthorized GM papaya in imported processed papaya food. Transgenic vector construct that provides resistance to the PRSV strains isolated in Thailand was detected. An original and specific real-time polymerase chain reaction method was generated to qualitatively detect the PRSV-Thailand-resistant GM papaya.

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