Synthesis of Clovamide Analogues that Inhibit NO Production in Activated BV-2 Microglial Cells

Abstract

A series of methyl ester of clovamide analogues, where the hydroxyl group of catechol moiety in caffeic acid and L-dopa was replaced with various functional groups, were synthesized and their inhibitory effects on nitric oxide (NO) production and inducible NO synthase expression in lipopolysaccharide-induced BV2 cells were tested. Among the synthesized compounds, 3,5-ditrifluoromethyl analogue 9l (IC₅₀ = 2.8 μM) exhibited a potency about 26.3 times greater than that of the parent compound 9a (IC₅₀ = 73.6 μM) and suppressed NO production dose-dependently without cytotoxicity. Compound 9l also inhibited iNOS expression in LPS-induced BV2 cells at 2.5, 5 and 10 μM concentrations. These results suggested that the dihydroxyl group of catechol moiety in caffeic acid unit is not essential for the suppression of NO production and that 9l has potential as a potent inhibitor of NO production.