Mitochondria-Selective Reduction of 62Cu-Pyruvaldehyde Bis(N4-methylthiosemicarbazone) (62Cu-PTSM) in the Murine Brain; a Novel Radiopharmaceutical for Brain Positron Emission Tomography (PET) Imaging

Yasuhisa FUJIBAYASHI; Kouichi WADA; Hideyuki TANIUCHI; Yoshiharu YONEKURA; Junji KONISHI; Akira YOKOYAMA

doi:10.1248/bpb.16.146

Mitochondria-Selective Reduction of ⁶²Cu-Pyruvaldehyde Bis(N⁴-methylthiosemicarbazone) (⁶²Cu-PTSM) in the Murine Brain; a Novel Radiopharmaceutical for Brain Positron Emission Tomography (PET) Imaging

Yasuhisa FUJIBAYASHI, Kouichi WADA, Hideyuki TANIUCHI, Yoshiharu YONEKURA, Junji KONISHI, Akira YOKOYAMA

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Keywords: ^<62>Cu-PTSM, brain, retention mechanism, perfusion imaging, mitochondria, reduction

JOURNAL FREE ACCESS

1993 Volume 16 Issue 2 Pages 146-149

DOI https://doi.org/10.1248/bpb.16.146

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Abstract

The retention mechanism of ⁶²Cu-pyruvaldehyde bis(N⁴-methylthiosemicarbazone) (⁶²Cu-PTSM) in the murine brain was evaluated. For this purpose, stable Cu-PTSM was subjected to electron spin resonance spectrometry (ESR) and high performance liquid chromatography (HPLC) analysis to determine the valence state, coordination structure and tissue metabolism. In murine brain homogenate, ESR and HPLC analysis indicated the reduction and cleavage of Cu(II)-PTSM to Cu(I). This virtually irreversible reduction was specifically initiated by the mitochondrial enzymatic system in the murine brain.

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