A Convenient Screening Test for Hypoxic Cell Radiosensitizers/Cytotoxins

Hitoshi HORI; Hideakira YOKOYAMA; Hideko NAGASAWA; Chieko MURAYAMA; Tomoyuki MORI; Syuji YONEI; Tetsuo SATOH; Seiichi INAYAMA

doi:10.1248/bpb.17.1676

Hitoshi HORI, Hideakira YOKOYAMA, Hideko NAGASAWA, Chieko MURAYAMA, Tomoyuki MORI, Syuji YONEI, Tetsuo SATOH, Seiichi INAYAMA

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Keywords: hypoxic cell radiosensitizer, hypoxic cell cytotoxin, radiosensitization, E. coli B/r screening test, EMT6/KU cell in vitro assay

JOURNAL FREE ACCESS

1994 Volume 17 Issue 12 Pages 1676-1678

DOI https://doi.org/10.1248/bpb.17.1676

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Abstract

A convenient in vitro screening test using E. coli B/r for evaluating a variety of hypoxic cell radiosensitizers/hypoxic cell cytotoxins has been developed for the initial selection of candidates in medicinal/organic chemistry laboratories. E. coli cells were used for convenience since : (1) the bacterium is grown using commercially available broths, where it multiplies repidly, and requires little specialized equipment for growth and handling. (2) More is known about the genetics and biochemistry of the radiation damage to these cells and their repair than any other organism.

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