Biochemical Studies of Inherited Diseases Related to Abnormal Cholesterol Metabolism. I. High-Performance Liquid Chromatographic Analysis of Bile Alcohol Glucuronides in Cerebrotendinous Xanthomatosis

Akira OHSHIMA; Taiju KURAMOTO; Takahiko HOSHITA

doi:10.1248/bpb.17.721

Akira OHSHIMA, Taiju KURAMOTO, Takahiko HOSHITA

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Keywords: HPLC, cerebrotendinous xanthomatosis, bile alcohol glucuronide, 5β-cholestane-3α, 7α, 12α, 25-tetrol 3-glucuronide

JOURNAL FREE ACCESS

1994 Volume 17 Issue 5 Pages 721-723

DOI https://doi.org/10.1248/bpb.17.721

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Abstract

We developed a rapid and simple quantitative method for measuring bile alcohol glucuronides in serum involving high-performance liquid chromatography without group separation and hydrolysis. The assay of 5β-cholestane-3α, 7α, 12α, 25-tetrol 3-glucuronide, the major bile alcohol component in serum from cerebrotendinous xanthomatosis patients, with the present method was useful for the diagnosis of cerebrotendinous xanthomatosis.

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