Inhibitory Effect of Some Oxygenated Stigmastane-Type Sterols on 12-O-Tetradecanoylphorbol-13-acetate-Induced Inflammation in Mice

Abstract

The oxygenated stigmastane-type sterols stigmastane-3β, 6α-diol, stigmastane-3β, 6β-diol, 7α-hydroxysitosterol and its diacetyl derivative, 7β-hydroxysitosterol and its diacetyl derivative, 7-oxositosterol, 4β-hydroxysitosterol, and stigmast-4-ene-3β, 6β-diol were evaluated with respect to their anti-inflammatory activity against 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced inflammation in mice. All of the sterols, with the exception of 7α-hydroxysitosterol and its diacetyl derivative, were found to possess marked inhibitory activity. The 50% inhibitory dose of these compounds for TPA-inflammation (1 μg) was 0.5-1.0 mg/ear.