Stability, Specific Binding Activity, and Plasma Concentration in Mice of an Oligodeoxynucleotide Modified at 5'-Terminal with Poly (ethylene glycol)

Takeo KAWAGUCHI; Hiroki ASAKAWA; Yasutaka TASHIRO; Kazuhiko JUNI; Toshihiko SUEISHI

doi:10.1248/bpb.18.474

Takeo KAWAGUCHI, Hiroki ASAKAWA, Yasutaka TASHIRO, Kazuhiko JUNI, Toshihiko SUEISHI

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Keywords: antisense, oligodeoxynucleotide, poly (ethylene glycol), in vivo, modification, stability

JOURNAL FREE ACCESS

1995 Volume 18 Issue 3 Pages 474-476

DOI https://doi.org/10.1248/bpb.18.474

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Abstract

Oligodeoxynucleotide (ODN) composed of 15 nucleotides was modified at 5'-terminal phosphate with hexylamine linker and chemically activated poly (ethylene glycol). This derivative showed improved characteristics in terms of enzymatic stability, binding activity, and in vivo retention in mouse. The data are discussed in comparison with those of corresponding unmodified and phosphorothioate ODNs.

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