Biological and Pharmaceutical Bulletin
Online ISSN : 1347-5215
Print ISSN : 0918-6158
A Combined Assay of Cell Vability and in Vitro Cytotoxicity with a Highly Water-Soluble Tetrazolium Salt, Neutral Red and Crystal Violet
Munetaka ISHIYAMAHideyuki TOMINAGAMasanobu SHIGAKazumi SASAMOTOYosuke OHKURAKeiyu UENO
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1996 Volume 19 Issue 11 Pages 1518-1520

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Abstract

Cell viability and in vitro cytotoxicity assay methods were developed using a combination of dyes, 4-[3-(4-iodophenyl)-2-(4-nitrophenyl)-2H-5-tetrazolio]-1, 3-benzene disulfonate (WST-1), neutral red (NR) and crystal violet (CV), with HeLa cells as a bioindicator. As WST-1 produces a highly water soluble and non-cytotoxic formazan dye, it allows each assay to be carried out in one culture dish. The combined cell viability assay using WST-1, NR and CV gave an absorbance that correlated linearly with the number of cells over the range 1000 to 50000 cells/well. The combined assay was applied to the evaluation of the IC50 values for sodium dodecyl sulfate as a model toxicant, which yielded similar values to those obtained with each assay independently.

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