Biological and Pharmaceutical Bulletin
Online ISSN : 1347-5215
Print ISSN : 0918-6158
ISSN-L : 0918-6158
Restriction Fragment Length Polymorphisms of rDNA and Variation of Essential Oil Composition in Atractylodes Plants
Hajime MIZUKAMIRyosuke SHIMIZUHiroshi KOHDAMareshige KOHJYOUMAFumiaki KAWANISHINoboru HIRAOKA
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1996 Volume 19 Issue 4 Pages 577-580

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Abstract

Total DNA was extracted from the leaves of Atractylodes lancea DE CANDOLLE, A. ovata DE CANDOLLE and A. japonica KOIDZUMI ex KITAMURA of various origins and hybridized with digoxigenin-labeled rice ribosomal DNA after digestion with eight different restriction endonucleases. The resulting restriction fragment length polymorphism (RFLP) profiles allowed us to distinguish the three Atractylodes species when DNA was digested with SacI. Although atractylon was detected in the rhizomes of some of the cultivated strains of A. lancea, their RFLP profiles clearly indicate that these plants are not hybrids of A. ovata or A. kaponica.RFLP analysis also revealed the presence of intraspecific variation in DNA sequence of rRNA locus among A. lancea as well as A. japonica.

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