Biological and Pharmaceutical Bulletin
Online ISSN : 1347-5215
Print ISSN : 0918-6158
ISSN-L : 0918-6158
Effects of Zinc and Copper on Uptake of Cadmium by LLC-PK1 Cells
Tetsuya ENDOOsamu KIMURAMasakatsu SAKATA
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1996 Volume 19 Issue 7 Pages 944-948

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Abstract
Effects on zinc (Zn) and copper (Cu) on cadmium (Cd) accumulation were investigated in LLC-PK1 cells. Coincubation with Zn or Cu decreased Cd uptake by the cells in a concentration-dependent manner. Cd accumulation data suggested that Cd is taken up by the cells via simple diffusion and carrier-mediated transport. Zn and Cu significantly increased the Km of Cd uptake with no or little effect on the Vmax. Pretreatment of the cells with ouabain and a metabolic inhibitor (FCCP) significantly decreased Cd accumulation, and coincubation with Zn or Cu led to an additional decrease in Cd accumulation by these cells. Overall, it seems that, in addition to simple diffusion, Cd is also taken up by LLC-PK1 cells via carrier-mediated mechanisms that are competitively inhibited by Zn and Cu.
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© The Pharmaceutical Society of Japan
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