Effects of Cytokine-Induced Neutrophil Chemoattractants (CINCs) on Shape Change, Adhesiveness and Phagocytosis of Rat Neutrophils

Abstract

Rat cytokine-induced neutrophil chemoattractants (CINC)-1, -2α, -2β and -3 belong to α-chemokines and are known to be potent chemoattractants for rat neutrophils in vivo and in vitro. In this study, the abilities of CINCs to affect several functions of rat neutrophils are estimated. All CINCs at a concentration of 10^-8 M induced the polarization of rat neutrophils. The adhesion of each CINC-treated neutrophil to fibrinogen significantly increased, reaching a maximal adhesion at 10^-8 M of each CINC. The CINCs enhanced the phagocytosis of heat-killed yeast cells by neutrophils in a dose-dependent manner. In contrast, CINCs did not induce the production of reactive nitrogen intermediates. The results suggest that CINCs play a significant role in neutrophil infiltration and phagocytosis at inflammatory sites in rats.