Biological and Pharmaceutical Bulletin
Online ISSN : 1347-5215
Print ISSN : 0918-6158
ISSN-L : 0918-6158
Virus Inactivation in Superoxide Dismutase Preparations by Ultraviolet Light Irradiation
Junichi HIRAYAMAHideki ABEKenji IKEBUCHIMotohiro HORIUCHIMorikazu SHINAGAWANaoki KAMOSadayoshi SEKIGUCHI
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1998 Volume 21 Issue 6 Pages 621-623

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Abstract

Viral inactivation in superoxide dismutase (SOD) derived from human red cells was carried out by ultraviolet light C (UVC) irradiation. With 400J/m2 UVC irradiation, the titer of canine parvovirus (CPV, a nonenveloped virus), M13 bacteriophage (M13, a nonenveloped phage) and vesicular stomatitis virus (VSV, an enveloped virus), which were spiked into SOD solution, were reduced by >4.6log10 (detection limit), 7.0log10 and 6.2log10, respectively. The SOD activity was maintained and the band pattern of SOD on an electrophoresis gel was not changed even by 1000J/m2 UVC irradiation. These results indicate that UVC irradiation is a promising method for the inactivation of both enveloped and nonenveloped viruses in SOD preparations while maintaining its activity.

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