1998 Volume 21 Issue 6 Pages 624-627
An unknown adenine-related compound (UKC) in rat liver mitochondria was characterized. Based on the sensitivity to periodate oxidation, nuclease P1 digestion, property of fluorescence derivatization, elution behavior on different separation modes of HPLC columns and the mass spectrum of purified UKC, the UKC was identified as adenosine 3'-monophosphate (3'-AMP), an intracellular P-site inhibitor of adenylate cyclase. 3'-AMP may be enzymatically produced from RNA in rat liver mitochondria in temperature- and time-dependent manners. A partial characterization of 3'-AMP forming enzyme is included.