Biological and Pharmaceutical Bulletin
Online ISSN : 1347-5215
Print ISSN : 0918-6158
ISSN-L : 0918-6158
S-(1, 2-Dicarboxyethyl)glutathione in Yeast : Partial Purification of Its Synthesizing Enzyme
Seiji TSUBOIKunihiko KIYONOBun-ichiro ONOTakahiro SAKAUEKazumi OGATA
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1999 Volume 22 Issue 1 Pages 21-25

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Abstract

S-(1, 2-Dicarboxyethyl)glutathione (DCE-GS) was found in Saccharomyces cerevisiae, but not in bacterial species nor in a unicellular alga (Acetabularia acetabulm). The enzyme that catalyzes condensation of L-malate and glutathione (GSH) to form DCE-GS was partially purified from baker's yeast. It had a molecular mass of 49 kDa and was monomeric and the Km values were 2.2 and 1.4 mM for L-malate and GSH, respectively. The enzyme had a pH optimum of 7.5. DCE-GS levels in yeast cells were significantly higher in aerobic cultures than in anaerobic ones. DCE-GS was synthesized in cells cultured between 20 and 35°C.

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