Biological and Pharmaceutical Bulletin
Online ISSN : 1347-5215
Print ISSN : 0918-6158
ISSN-L : 0918-6158
Role of Phospholipase D-Derived Phosphatidic Acid as a Substrate for Phospholipase A2 in RBL-2H3 Cells
Kazuyuki KITATANISatoshi AKIBATakashi SATO
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2000 年 23 巻 12 号 p. 1430-1433

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The implications of phospholipase D (PLD) in cytosolic phospholipase A2 (cPLA2) activation were studied in a mast cell line, RBL-2H3, upon stimulation with antigen. Antigen-stimulated prostaglandin D2 generation was apparently suppressed by ethanol with a concomitant decrease in phosphatidic acid (PA) formation. The prostaglandin D2 generation was also inhibited almost completely by methyl arachidonyl fluorophosphate (MAFP), an inhibitor of cPLA2, but not by diacylglycerol lipase inhibitor. Furthermore, stimulation with antigen resulted in an increase in lysophosphatidic acid formation, which was suppressed by MAFP in parallel with an increase in PA formation. These results suggest that PA formed by the catalytic action of PLD is used as a substrate for cPLA2. thus PLD regulates cPLA2 activation in antigen-stimulated RBL-2H3 cells.

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© The Pharmaceutical Society of Japan
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