微量カルバゾールの抽出-蛍光光度定量法
1985 年 34 巻 6 号 p. 371-373
詳細
1985 年 34 巻 6 号 p. 371-373
It was reported previously that the fluorescence of carbazole can be selectively measured by use of dimethyl sulfoxide (DMSO) containing potassium iodide, sodium methoxide, and methanol as a solvent. The interference of polynuclear aromatic compounds for fluorometric determination of carbazole could be reduced successfully by the employment of the following procedure. After a sample had been dissolved in 20ml of cyclohexane-xylene (1:2) mixture, the carbazole was extracted with 3ml of DMSO containing potassium iodide (0.30g/ml), sodium methoxide (1mg/ml), and methanol (2.0%). The DMSO phase was subjected to the measurement of fluorescence intensity. The results indicated that trace amounts of carbazole could be effectively separated from most of the interfering substances. The determination limit was found to be 20ng.
