電気クロマトグラフ法(第6報)

緩衝液のイオン濃度変化による移動度およびイオンの移動帯の長さの変化

抄録

The Changes of the Mobility of Ion and the Length of Band on the Filter Paper with the Change of Ionic Concentration of Buffer Solution.
The effect of mobility of Ba⁺⁺, Hg⁺⁺, Fe⁺⁺⁺ and Pb⁺⁺ in filter paper (Toyo filter paper No. 3 2×50cm) saturated with HCl-CH₃COONa-NaCl buffer solution is investigated by changing the concentration of NaCl within the range of 00.5 Mol; the mobility of Ba⁺⁺ remain practically unchanged by increasing the concentration of NaCl, that of Fe⁺⁺⁺ increased, that of Pb⁺⁺ decreased, while that of Hg⁺⁺ formed a complex ion with Cl^- and increased its mobility toward the anode. The effect of changing of cationic concentrations of buffer solution to O.040.265 Mol is investigated by measuring the average length of migration band of ions which are spotted on the paper each 0.04 cc of the concentration of O.4 equivalent per liter; a linear relationship is found between the reciprocal number of the cationic concentration in buffer solution and average length of migration band, in which the positions of straight lines of Ni⁺⁺, Co⁺⁺, Ba⁺⁺, and Al⁺⁺⁺ are found in approximately the same and their average value is expressible in a straight line: y≅5/c+16, where y=average length in mm of migration band; C=cationic concentration in Mol in buffer solution. By using Ba⁺⁺ as a sample, the relation between ionic concentration of Ba⁺⁺ and average length of migration band is investigated and found that the relationship is expressible in a straight line under a definite condition; it is possible to estimate a small amount of Ba⁺⁺ from an average length of migration band.