The Study on the Selection of Bacterial Strain suitable for an Acid-Producing Process of Two-Step Methane Fermentation by Separation Culture

Abstract

Bacterial strains were cultured by separation in an acid-producing process in order to improve the efficiency of two step methane fermentaion. Separated strains were cultivated a pure culture, among which effective strains were selected based on the comparison of proliferating and metabolizing ability. As a result, thirty-eight separated strains were obtained in the acid production process. Moreover, No.24 and No.28 were superior strains compared to other strains as shown by the comparison of pure culture of separated strains. Therefore, this study determines that No.24 and No.28 were effective bacterial strains suitable for the acid-producing process of two-step methane fermentaion.