抄録
To get a pure culture of the cellulose-decomposing bacteria, there has been devised 1% agar plate composed of K2HPO4(1.0g), CaCO3(2.0g), MgSO5(0.5g), (NH4)2SO4(1.0g), NaCl(trace), distilled water (1000cc), and agar (1.0g). In this method, cellulose is not used, but the cellulose-decomposing bacteria and the other bacteria form colonies. In dilute methodpure culture is obtained. When inoculated on cellulose, these cellulose-decomposing bacteria, which are obtained by this method, do not diminish their activity, In this agar plate, the mesophilic cellulose-decomposing bacteria and also the thermophilic cellulose-decomposing bacteria are cultured, but the latter is rarely seen. On this point fnrther investigation will be continued. It has been found in the first experiment that by using the specific agar after culturing in OMELIANSKY medium, a pure culture of the cellulose-decomposing bacteria were obtained in a compatively short time. Shortening of the isolation stadium, is due to the apply of the specific agar, but not the apply of OMELIANSKY medium. It takes about 30-50 days to get a pure culture, but if OMELIANSKY stadium will be shortened, the isolation will become more easy. For this purpose, it has been explained in the second experiment that carbohydrate is a good stimulus to cellulose-decomposition. Especially pentose group and some of hexose group are found excellent.
